Composite

Part:BBa_K4229048:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)


"Full wiffelball" without tags, under regulation of lambdapLhybrid promotor and LacI promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1519
    Illegal NotI site found at 1323
    Illegal NotI site found at 2700
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 444
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1519
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 2320
    Illegal NgoMIV site found at 2676
    Illegal AgeI site found at 1127
    Illegal AgeI site found at 1235
    Illegal AgeI site found at 1822
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The plasmid containing this protein was kindly sent to us by the Kerfeld group.

References

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.

H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.